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KMID : 0861020180330020053
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Korea Journal of Herbology
2018 Volume.33 No. 2 p.53 ~ p.58
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The effect of Plantaginis asiaticae Folium on lipid accumulation in 3T3-L1 adipocytes by extraction conditions
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Jeong Mi-Jin
Kim So-Young
Kim Yoo-Jin
Lee Un-Tak
Choo Sung-Tae
Kim Han-Hyuk
Kim Mi-Ryeo
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Abstract
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Objectives : Plantaginis asiaticae Folium (PA) has been widely used in Korean medicine for treatment of liver disease, stomach troubles and inflammation. We investigated the effect of PA on lipid accumulation in 3T3-L1 according to extraction conditions.
Methods : The MTT assay was employed to evaluate the cytotoxicity of PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) on 3T3-L1 preadipocytes. Oil red O staining was used to identify intracellular lipid accumulation in 3T3-L1. 3T3-L1 adipocytes were treated with PA at concentration ranging in 0.1, 0.2 and 0.4 ㎎/㎖. PA was extracted by different extraction conditions such as extraction solvents, extraction time, and extraction temperature. In addition, UPLC analysis was used for determination of candidates of active ingredients in PA.
Results : 3T3-L1 preadipocytes were treated with PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) and there was no cytotoxicity. Oil red O staining was employed to identify the effect of PA on lipid accumulation in 3T3-L1. In the present study, PA water extraction at 70℃ for 6 hours decreased greatly in lipid accumulation. The range of concentrations was 0.1, 0.2 and 0.4 ㎎/㎖. Concentration at 0.2 ㎎/㎖ was the most effective one among them. Candidates of active ingredients in PA were shown plantamajoside and acteoside through UPLC.
Conclusions : These results suggest that the effect of PA water extraction at 70℃ on lipid accumulation in 3T3-L1 is superior to other extraction conditions. We suppose that plantamajoside and acteoside may be candidates of active ingredients in PA.
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KEYWORD
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Plantaginis asiaticae Folium, Lipid accumulation, 3T3-L1 cell, Cell viability, Water extract, Extraction conditions
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